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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative <t>images</t> of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G <t>Western</t> <t>blot</t> of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)
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Image Search Results


Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative images of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G Western blot of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)

Journal: Cell & Bioscience

Article Title: Combination of GLP-1 receptor agonist and Akkermansia muciniphila Akk11 reduces adiposity and ameliorates MASLD in T2D mice

doi: 10.1186/s13578-025-01525-4

Figure Lengend Snippet: Oral administration of AKK11 enhances the metabolic benefits of GLP-1RA in db/db mice. A Representative images of mice of GLP-1RA and GLP-1RA + AKK groups, B , C Representative images ( B ) and weights ( C ) of eWAT, iWAT and BAT from mice of GLP-1RA and GLP-1RA + AKK groups, N = 6, D – E H&E staining ( D ) and average cell area ( E ) of iWAT from GLP-1RA and GLP1RA + AKK group mice, bar = 50 μm, N = 3, F–G Western blot of PPARα and FASN protein expression levels in the iWAT ( F ) and eWAT ( G ) of each group, N = 3, H The TG level in the serum, N = 8,6 for each group ( I – G ), Concentration of LPS ( I ) and GLP-1 ( G ) in serum of mice in all groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)

Article Snippet: After washing three times with TBST, the membranes were incubated with secondary anti- bodies (Jackson) for 1 h at 37 °C and then quantitative western blot images were recorded with a fully automated chemiluminescence image analysis system (Clinx).

Techniques: Staining, Western Blot, Expressing, Concentration Assay

GLP-1RA and AKK co-treatment inhibits hepatic lipogenesis and ameliorates MASLD in db/db mice. A , B Representative images ( A ) and weights ( B ) of liver from GLP-1RA and GLP-1RA + AKK group, N = 6, C H&E, Sirius Red, Masson’s Trichrome, and Oil Red staining of mice liver sections in WT, DB, GLP-1RA and GLP-1RA + AKK group, bar = 50 μm, D Fibrotic area in mouse liver Sirius Red staining, N = 3, E Relative area of lipid droplets in mouse Oil Red O staining, N = 3, F GO enrichment of all overlapping DEGs from liver of GLP-1RA + AKK group compared to GLP-1RA group, N = 3, G Heatmap of DEGs enriched in fatty acid metabolism and cholesterol metabolism from liver of each group, N = 3, H Relative mRNA levels of in liver from GLP-1RA and GLP-1RA + AKK group, N = 3, I – L Western blot and gray area analysis of ACLY (I), FASN ( J ) and MFN2 ( K ) protein expression levels in the liver of GLP-1RA and GLP-1RA + AKK groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)

Journal: Cell & Bioscience

Article Title: Combination of GLP-1 receptor agonist and Akkermansia muciniphila Akk11 reduces adiposity and ameliorates MASLD in T2D mice

doi: 10.1186/s13578-025-01525-4

Figure Lengend Snippet: GLP-1RA and AKK co-treatment inhibits hepatic lipogenesis and ameliorates MASLD in db/db mice. A , B Representative images ( A ) and weights ( B ) of liver from GLP-1RA and GLP-1RA + AKK group, N = 6, C H&E, Sirius Red, Masson’s Trichrome, and Oil Red staining of mice liver sections in WT, DB, GLP-1RA and GLP-1RA + AKK group, bar = 50 μm, D Fibrotic area in mouse liver Sirius Red staining, N = 3, E Relative area of lipid droplets in mouse Oil Red O staining, N = 3, F GO enrichment of all overlapping DEGs from liver of GLP-1RA + AKK group compared to GLP-1RA group, N = 3, G Heatmap of DEGs enriched in fatty acid metabolism and cholesterol metabolism from liver of each group, N = 3, H Relative mRNA levels of in liver from GLP-1RA and GLP-1RA + AKK group, N = 3, I – L Western blot and gray area analysis of ACLY (I), FASN ( J ) and MFN2 ( K ) protein expression levels in the liver of GLP-1RA and GLP-1RA + AKK groups, N = 3. Data represent mean ± SEM (Unpaired Student’s t-test for two group, one-way ANOVA and Tukey’s post hoc test for four group, * p < 0.05; ** p < 0.01; *** p < 0.001)

Article Snippet: After washing three times with TBST, the membranes were incubated with secondary anti- bodies (Jackson) for 1 h at 37 °C and then quantitative western blot images were recorded with a fully automated chemiluminescence image analysis system (Clinx).

Techniques: Staining, Western Blot, Expressing